We investigate the impact of the recently released FNAL-E906 (SeaQuest) data on the ratio of proton-deuteron and proton-proton Drell-Yan production cross-sections on the sea quark PDFs. We find that they have constraining power on the light-quark sea isospin asymmetry $(\bar{d}-\bar{u})(x)$ and on the $(\bar{d}/\bar{u})(x)$ ratio at large longitudinal momentum fraction $x$ values,
and that their constraints turn out to be compatible with those from Drell-Yan data in collider experiments (Tevatron and Large Hadron Collider) and in old fixed-target experiments by the FNAL-E605 and FNAL-E866 collaborations. We study the impact of nuclear corrections due to the deuteron target, finding them within 1% in most of the kinematic region covered by SeaQuest. We perform a new proton PDF fit, including SeaQuest data, using the ABMP16 methodology and we compare it to the ABMP16 baseline.