Motivated by the process of reconstructing real-time spectral functions from Euclidean correlators in lattice QCD, we derive explicit expressions for the fermionic determinant and the propagator of the four-dimensional clover-improved Wilson-Dirac fermions on anisotropic lattices in terms of the three-dimensional Wilson-Dirac Hamiltonian operator. We derive an effective Hamiltonian that governs Euclidean time evolution at finite temporal lattice spacing, and demonstrate its Hermiticity and particle-anti-particle symmetry. Our expressions allow to quantify lattice artifacts of the spectral reconstruction procedure on Euclidean correlators with finite lattice spacing.